| COLUMBIA COUNTY SCHOOL SYSTEM | Government | 1389 |
| VA MEDICAL CENTER | Hospitals/Health Services/Imaging/Labs | 970 |
| SITEL | Computer/Internet-Consultants/Sales/Service | 750 |
| TIMCO-LAKE CITY | Aircraft Maintenance | 575 |
| WAL -MART SUPERCENTER | Department Stores/Shopping Centers | 539 |
| SHANDS LAKE SHORE | Hospitals/Health Services/Imaging/Labs | 433 |
| PCS PHOSPHATE | Manufacturer | 315 |
| ANDERSON COLUMBIA CO., INC. | Asphalt/Paving | 305 |
| CITY OF LAKE CITY | Government | 260 |
| CITY OF LAKE CITY | Government | 259 |
| CCA-LAKE CITY CORRECTIONAL FACILITY | Correctional Facility | 258 |
| COLUMBIA COUNTY MANAGER | Government | 215 |
| LAKE CITY COMMUNITY COLLEGE | Colleges/University | 203 |
| SCAFF'S INC. - S&S FOOD STORES | Convenience Stores | 187 |
| CORBITT MANUFACTURING CO., INC. | Manufacturer | 170 |
| DICKS ENTERPRISES | Land Development | 132 |
| HEALTH CENTER OF LAKE CITY | Hospitals/Health Services/Imaging/Labs | 130 |
| SUWANNEE VALLEY 4 C'S, INC. | Child Care | 116 |
| NEW MILLENNIUM | Manufacturer | 101 |
| TOWNHOMES, LLC | Manufacturer | 100 |
| HUNTER PANELS, INC. | Manufacturer | 53 |
| PUBLIX SUPER MARKETS, INC. | Grocery Stores | 50 |
| LAKE CITY COMMUNITY COLLEGE | Colleges/University | 2 |